Part:BBa_J435362

BN1_BCD2_DsbA

Riboj is a ribozyme that reduces DNA context from expression. Additionally contains DsbA tag. Defined as a BN1 part.

FLANKED 5' fusion site: TACT, 3' fusion site: CCAT

Source organism: N/A, Target organism: Escherichia coli

Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003391.html

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 564
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 564
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 352
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 564
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 564
  Illegal AgeI site found at 184
  
• 1000
  COMPATIBLE WITH RFC[1000]