Part:BBa_J428374

pJUMP49-2D' SpecR Type IIS Level 2 vector for D modules

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 2991
Illegal suffix found at 16
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2991
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NotI site found at 24
Illegal NotI site found at 2997
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2991
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 2991
Illegal suffix found at 17
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 2991
Plasmid lacks a suffix.
Illegal XbaI site found at 3006
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NgoMIV site found at 1039
Illegal NgoMIV site found at 1441
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 3013
Illegal BsaI.rc site found at 10
Illegal SapI.rc site found at 2239