Plasmid_Backbone

Part:BBa_J428366

Designed by: Marcos Valenzuela-Ortega   Group: 2021 Engineering Committee   (2022-06-15)


pJUMP42x-2A SpecR Type IIS Level 2 vector. Origin RK2 (broad-host-range).

pJUMP42x-2A SpecR Type IIS Level 2 vector. Origin RK2 (broad-host-range). *CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3772
    Illegal suffix found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3772
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 3778
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3772
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3772
    Illegal suffix found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3772
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3787
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1039
    Illegal NgoMIV site found at 1441
    Illegal NgoMIV site found at 2326
    Illegal NgoMIV site found at 3437
    Illegal NgoMIV site found at 3505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3794
    Illegal BsaI.rc site found at 10


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/jump/level2
//plasmidbackbone/assembly/typeiis
Parameters
resistanceSpectinomycin