Plasmid_Backbone
Part:BBa_J428350
Designed by: Marcos Valenzuela-Ortega Group: 2021 Engineering Committee (2022-06-15)
pJUMP26-1A KanR Type IIS Level 1 vector. Origin p15A (medium copy number)
pJUMP26-1A KanR Type IIS Level 1 vector. Origin p15A (medium copy number)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 2296
Illegal suffix found at 16 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2296
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NotI site found at 24
Illegal NotI site found at 2302 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2296 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2296
Illegal suffix found at 17 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2296
Plasmid lacks a suffix.
Illegal XbaI site found at 2311
Illegal SpeI site found at 17
Illegal PstI site found at 31
Illegal NgoMIV site found at 1390
Illegal AgeI site found at 1828 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//collections/jump/level1
//plasmidbackbone/assembly/typeiis
//collections/jump/level1
//plasmidbackbone/assembly/typeiis
insert | sfGFP |
resistance | kanamycin |