Coding
CmR Backwa
Part:BBa_J31004
Designed by: Erin Zwack, Sabriya Rosemond Group: iGEM06_Davidson (2006-07-12)
chloramphenicol acetyltransferase (backwards, CmB) [cf. BBa_J31005]
Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance. PLEASE READ THE NOTE ABOUT THIS PART'S BIOBRICKS ON THE PART DESIGN PAGE.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 177
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 343
[edit]
Categories
Parameters
//cds/enzyme
//cds/selectionmarker/antibioticresistance
//cds/selectionmarker/antibioticresistance
ec_num | 2.3.1.28 |
kegg | none |
protein | CAT-bkw |
swisspro | P62577 |