Coding
CmR Backwa

Part:BBa_J31004

Designed by: Erin Zwack, Sabriya Rosemond   Group: iGEM06_Davidson   (2006-07-12)

chloramphenicol acetyltransferase (backwards, CmB) [cf. BBa_J31005]

Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance. PLEASE READ THE NOTE ABOUT THIS PART'S BIOBRICKS ON THE PART DESIGN PAGE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 343


[edit]
Categories
//cds/enzyme
//cds/selectionmarker/antibioticresistance
Parameters
ec_num2.3.1.28
keggnone
proteinCAT-bkw
swissproP62577