RBS

Part:BBa_J119435

Designed by: Raechel Tittor, Ali Tauchen   Group: Eckdahl Lab   (2019-07-10)


BCD16 with TA

BCD16 (Bicistronic Design) is a sequence of two ribosome binding sites that promotes higher gene expression originally designed by Drew Endy and has a missing TA added into the T7 promoter. The BCD was cloned into K199176, allowing for gene expression using a T7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731


[edit]
Categories
Parameters
None