RBS

Part:BBa_J119393

Designed by: Anthony J. Eckdahl   Group: Eckdahl Lab   (2016-04-29)

Collection of Bicistronic RBSs from N8 rClone Red Library

Oligonucleotides that encoded Bicistronic RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red (BBa_J119384). to produce a library of 65,536 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.

N8CdRBS.PNG


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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