RBS

Part:BBa_J100458

Designed by: Eleanor McCall   Group: Campbell M Lab   (2018-10-23)


Even More As

Our research team has designed an RBS in an attempt to stimulate more translation of mScarlet protein in E. coli transformed with our plasmid. We hypothesized that weaker bonds formed by non Watson-Crick base pairings, specifically A-C pairings, will facilitate the movement of mRNA through the ribosome and increase translation. After examining results from the first iteration of the experiment (BBa_J100439) and exploring the efficiency of folding in mRNA structure, we have made several changes to the RBS to correct errors and avoid folding. Compared to our oligo from the first experiment (BBa_J100439), we moved our 11 base-pair RBS element to directly upstream of the GCGG right sticky end. In addition, we changed one of those 11 bases and one of the 8 preceding bases from G to A, increasing our total of non-Watson Crick base-pairs to 3 in the RBS and 4 in the total sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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