RNA

Part:BBa_J100085

Designed by: Caroline Vrana   Group: Campbell M Lab   (2012-07-13)

short CRISPR sequence with GFP target spacer

The CRISPR system (Clustered Regularly Interspaced Short Palindromic Repeats) has been shown to act as a bacterial immune system. This system is characterized by short repeats, called CRISPR repeats, and in between these repeats are short, unique segments of DNA that have been acquired from foreign DNA that particular bacteria or archaea has encountered. E.coli, as well as other bacteria and archaea can use these "target spacer" segments to attack and destroy the foreign objects that contain that DNA, thereby functioning as an immune system. This part is an engineered synthetic CRISPR system to be used in E.coli. The one target spacer is a short, 30 bp sequence that targets the GFP gene (part E0040). This part also includes a BamHI restriction enzyme recognition site, for use in expanding this sequence if needed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 220
    Illegal XbaI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 221
    Illegal PstI site found at 235
    Illegal NotI site found at 6
    Illegal NotI site found at 228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 214
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 221
    Illegal XbaI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 221
    Illegal PstI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None