Part:BBa_J04685

Antiswitch enabling production of EYFP ("On" switch)

The presence of theophylline allows translation of mRNA transcribed from Biobrick BBa_E0032. No theophylline present causes repression of the coding region of BBa_E0032.

Usage and Biology

The antiswitch acts as an allosteric riboregulator, that in this case prevents translation of a target mRNA by attaching 3 bp upstream of the start codon of the target sequence, inhibiting translation of the coding region. When theophylline is present, the antiswitch releases from the mRNA and translation can proceed as normal. The target sequence for this antiswitch is: uagauggugagcaag. The plasmid containing this part is Blue Heron pUCminusMCS, which itself also expresses ampicillin resistance.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 94
  Illegal XbaI site found at 119
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 94
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 94
  Illegal BamHI site found at 55
  Illegal XhoI site found at 208
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 94
  Illegal XbaI site found at 119
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 94
  Illegal XbaI site found at 119
  
• 1000
  COMPATIBLE WITH RFC[1000]