Part:BBa_I746104

P2 promoter in agr operon from S. aureus

The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the activity of agr P2 promoter about 50-fold.

The cell density at which the agr system is activated is determined by the AgrA-independent activity of P2 promoter

The basal agr P2 promoter activity is high enough to make stochastic effects negligible. (E. Gustafsson et al. 2004)

We have tested the basal activity of P2 in E.coli by putting it with a reporter gene (GFP, E0840)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]