Part:BBa_I742161

crtI (phytoene dehydrogenase) and crtB (phytoene synthase)

crtIB genes encoding phytoene dehydrogenase and phytoene synthase from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Note: the crtI coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the mutated gene has not been confirmed. This version still contains the two PstI sites. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 500
  Illegal PstI site found at 911
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 500
  Illegal PstI site found at 911
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 93
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 500
  Illegal PstI site found at 911
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 500
  Illegal PstI site found at 911
  Illegal NgoMIV site found at 2061
  Illegal NgoMIV site found at 2191
  
• 1000
  COMPATIBLE WITH RFC[1000]