Regulatory
Pnot(O66)

Part:BBa_I732206

Designed by: Zhan Jian   Group: iGEM07_USTC   (2007-10-14)

NOT Gate Promoter Family Member (D001O66)

Promoter family member with symmetric operator O66 placed at +10.5. [http://2007.igem.org/USTC/HybridOperator#Hybrid_Operators_for_Weaker_Binding Used to weaken the binding strength of Repressor/Operator Pairs]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 18


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter/logic/ustc
//regulation/negative
Parameters
negative_regulators1
positive_regulators