Device
Rec-Open

Part:BBa_I726081

Designed by: Bangalore iGEM   Group: iGEM07_Bangalore   (2007-07-04)

Back to Bangalore iGEM 2007 Team Parts


Rec-LRY.RC

pL.luxR.Y::pR.C


Usage and Biology

IPTG drives the expression of the LuxR protein (monitored using polycistronic YFP). LuxR, when bound to externally supplied AHL, drives the expression of CFP. This device must be used in conjunction with an AHL sender device. It has been tested with [Sen-TIC].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1754


Functional Parameters

Device datasheet: Rec-LRY.RC

System datasheet: Sen-TIC+Rec-LRY.RC

[edit]
Categories
//function/cellsignalling
//function/regulation/transcriptional
Parameters
None