Coding
PhzS

Part:BBa_I723025

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)

PhzS

This is the coding region for the second of two Pseudomonas aeruginosa PA01 enzymes required to convert phenazine-1-carboxylic acid (PCA) into pyocyanin.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Janusan Jeyananthan
  • Summary: Added information collated from existing scientific studies

Enzyme catalytic activity: 5-methyl-phenazine-1-carboxylate + 2 H+ + NADH + O2 = CO2 + H2O + NAD+ + pyocyanin [1]

PhzS Reaction Diagram.JPG

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 28
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 76
    Illegal NgoMIV site found at 150
    Illegal NgoMIV site found at 283
    Illegal AgeI site found at 382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 385
    Illegal SapI.rc site found at 1146


  1. ↑ "phzS" n.d Uniprot. Accessed October 3, 2021. https://www.uniprot.org/uniprot/Q9HWG9
[edit]
Categories
//function/biosynthesis/pyocyanin
Parameters
None