Coding

Part:BBa_I716152

Designed by: Kristin Doan   Group: iGEM07_Berkeley_UC   (2007-06-28)


hemA (from CFT703)

This is an enzyme involved in the heme biosynthetic pathway. Also called Delta-aminolevulinate synthase

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Janusan Jeyananthan
  • Summary: Added information collated from existing scientific studies

Enzyme hemA catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA). When NADPH is absent, hemA exhibits substrate esterase activity, which causes the release of glutamate from tRNA.

Enzyme catalytic activity: (S)-4-amino-5-oxopentanoate + NADP+ + tRNAGlu = H+ + L-glutamyl-tRNAGlu + NADPH [1]

HemA.JPG

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ "hemA" n.d Uniprot. Accessed October 3, 2021. https://www.uniprot.org/uniprot/P0A6X1
[edit]
Categories
//function/biosynthesis/heme
Parameters
None