DNA

Part:BBa_I50050

Designed by: Reshma Shetty   Group:   (2006-02-03)


R6K replication origin

R6K minimal origin of replication. Only contains gamma origin and requires pir+ strain for replication. Copy number is dependent on amount of pir protein in the cell.


Usage and Biology

See also EZ-Tn5† <R6K&#947;ori /KAN-2> Sequence at http://www.epibio.com/sequences/R6Kori_Kan2.asp and general information at http://www.frankwu.com/R6K.html.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid/component/origin
Parameters
None