DNA

Part:BBa_I50001

Designed by: Reshma Shetty   Group:   (2006-03-11)


F plasmid backbone with BioBrick sites removed, reverse

This is a (near) single copy origin derived from the F plasmid. The naturally occurring BioBricks sites were removed with an intent to preserve the amino acid and codon frequency where possible. Similar to the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering. This part is a reverse copy of BBa_I50000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2436
    Illegal BglII site found at 4503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 30
    Illegal NgoMIV site found at 3946
    Illegal AgeI site found at 1699
    Illegal AgeI site found at 2592
    Illegal AgeI site found at 3822
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 845
    Illegal SapI.rc site found at 1842
    Illegal SapI.rc site found at 3052


[edit]
Categories
//plasmid/component/origin
Parameters
None