Signalling

Part:BBa_I13205

Designed by: Barry Canton   Group: Antiquity   (2004-07-30)


HSL/aiiA test construct

LuxR receiver circuit with an inducible promoter controlling aiiA production downstream.

Useful to measure the ability of aiiA (LVA+) to turn off a pLuxL driven receiver circuit in the presence of exogenous HSL

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3031
    Illegal AgeI site found at 3387
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal SapI site found at 3013


[edit]
Categories
//function/cellsignalling
Parameters
None