Part:BBa_G1003

Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

The primer sequence that binds to the BioBrick prefix for PCR amplification of BioBrick plasmid backbones. This sequence is used by Meagan Lizarazo at the Registry.

Note: this part has a typo and should instead be: tctagaagcggccgcgaattc. (Julie Norville)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 16
  Illegal XbaI site found at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 16
  Illegal NotI site found at 8
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 16
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 16
  Illegal XbaI site found at 1
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 16
  Illegal XbaI site found at 1
  
• 1000
  COMPATIBLE WITH RFC[1000]