Primer
Suffix-F

Part:BBa_G1002

Designed by: Reshma Shetty   Group: iGEM07_Example   (2008-10-17)


Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

The primer sequence that binds to the BioBrick suffix for PCR amplification of BioBrick plasmid backbones. This sequence is used by Meagan Lizarazo at the Registry.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//primer/plasmid/amplification
Parameters
directionF
len_end
len_fr
len_start
pct_gc65.0
targetBioBrick plasmid backbones
t_m62.1