Primer
SP6
Part:BBa_G00109
Designed by: Reshma Shetty Group: iGEM07_Example (2008-10-17)
SP6 promoter sequencing primer, 24-mer
This primer is complementary to a portion of the SP6 RNA Polymerase promoter and can be used to sequence DNA inserted in the polylinker regions of common cloning vectors.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//primer/SP6
direction | F |
len_end | |
len_fr | |
len_start | |
pct_gc | 37.5 |
target | SP6 promoter |
t_m | 50.5 |