File:BS3 GFP + AvrBS3.jpg

Revision as of 17:38, 10 October 2014 by Cara.deal (Talk | contribs) (A Nicotiana benthamiana leaf under UV light following infiltration (red circles) with Agrobacterium tumefaciens carrying plasmids encoding BS3_GFP_ocs (B) and BS3_GFP_ocs + 35s_AvrBS3_nos (A) respectively. There is GFP expression in the area infiltrated )

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A Nicotiana benthamiana leaf under UV light following infiltration (red circles) with Agrobacterium tumefaciens carrying plasmids encoding BS3_GFP_ocs (B) and BS3_GFP_ocs + 35s_AvrBS3_nos (A) respectively.

There is GFP expression in the area infiltrated with the multigene construct containing both the BS3 promoter and the TALE AvrBS3, showing that the promoter is active (A). In the area in which the infiltrated agrobacterium was just carrying the transcriptional unit encoding the promoter, GFP and terminator, there is no expression of GFP as the BS3 promoter is not active (B). This shows the function of AvrBS3 in activating the inducible BS3 promoter.

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current17:38, 10 October 2014Thumbnail for version as of 17:38, 10 October 20142,672 × 3,032 (2.65 MB)Cara.deal (Talk | contribs)A Nicotiana benthamiana leaf under UV light following infiltration (red circles) with Agrobacterium tumefaciens carrying plasmids encoding BS3_GFP_ocs (B) and BS3_GFP_ocs + 35s_AvrBS3_nos (A) respectively. There is GFP expression in the area infiltrated
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Camera manufacturer NIKON CORPORATION
Camera model NIKON D4
Exposure time 6/1 sec (6)
F Number f/16
ISO speed rating 1,250
Date and time of data generation 11:38, 2 October 2014
Lens focal length 60 mm
Width 4,928 px
Height 3,280 px
Compression scheme Uncompressed
Pixel composition RGB
Image data location 19,608
Number of components 3
Number of rows per strip 3,280
Bytes per compressed strip 48,491,520
Horizontal resolution 300 dpi
Vertical resolution 300 dpi
Data arrangement chunky format
Software used Adobe Photoshop Camera Raw 8.5 (Macintosh)
File change date and time 11:49, 2 October 2014
Exposure Program Manual
Exif version 2.3
Date and time of digitizing 11:38, 2 October 2014
APEX shutter speed 1.0E-6
APEX aperture 8
APEX exposure bias 1
Maximum land aperture 3.4 APEX (f/3.25)
Metering mode Pattern
Light source Unknown
Flash Flash did not fire
DateTimeOriginal subseconds 80
DateTimeDigitized subseconds 80
White balance Manual white balance
Digital zoom ratio 1
Focal length in 35 mm film 60 mm
Contrast Normal
Saturation Normal
Sharpness Normal