Part:BBa_K1467205:Experience

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Applications of BBa_K1467205

NRP-UEA iGEM 2014

This part was used by iGEM14_NRP-UEA-Norwich in The Green Canary Project and functioned in a multi-gene construct to induce activity of the BS3 promoter. In constructs where the TALE is present in conjunction with the BS3 promoter, the promoter is activated and drives transcription of the reporter. The presence of the AvrBS3 TALE effector represents infection of the plant by a pathogen.

Figure 1: A Nicotiana benthamiana leaf under UV light following infiltration (red circles) with Agrobacterium tumefaciens carrying plasmids encoding BS3_GFP_ocs (B) and BS3_GFP_ocs + 35s_AvrBS3_nos (A) respectively.

There is GFP expression in the area infiltrated with the multigene construct containing both the BS3 promoter and the TALE AvrBS3, showing that the promoter is active (A). In the area in which the infiltrated agrobacterium was just carrying the transcriptional unit encoding the promoter, GFP and terminator, there is no expression of GFP as the BS3 promoter is not active (B). This shows the function of AvrBS3 in activating the inducible BS3 promoter.

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