Chassis/B.subtilis key parts

Revision as of 01:50, 30 October 2008 by Jec105 (Talk | contribs) (→‎Multi-host vector pTG262)


B.subtilis strains B. subtilis chassis characterisation B. subtilis specific parts Key Parts and Devices

Bacillus subtilis Key Parts

This page is aimed to highlight some of the key biobricks for B. subtilis that are available on the Registry of Standard Biological Parts.

AmyE Integration Biobricks

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The AmyE integration bricks (BBa_K143001 and BBa_K143002) are composed of two biobricks that can be added to the 5' and 3' ends of a construct to allow integration into the B. subtilis genome. These biobricks have been successfully used within the constructs BBa_K143079 and BBa_K143082for integration. The possible advantages of integration are within stability and control of copy number.

Constitutive Promoters

TestingPromoters.PNG

The pVeg promoter and spoVG ribosome binding site (RBS) Biobrick (BBa_K143053) has been characterised for expression of both GFPmut3b (BBa_E0040) and mRFP1 (BBa_E1010). This data not only shows the parts are both working but once further parts have been characterized provides a basis for comparison. Further promoter and RBS combinations are currently being characterized, in addition to the construction of calibration curves to normalise the units of fluorescence to molecules of fluorescent proteins.

Multi-host vector pTG262

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