Difference between revisions of "Chassis/B.subtilis key parts"
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| − | The pVeg promoter and spoVG Biobrick ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K143053 BBa_K143053]) has been characterised for expression of both GFPmut3b ([https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040]) and mRFP1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010]). This data not only shows the parts are both working but once further parts have been | + | The pVeg promoter and spoVG ribosome binding site (RBS) Biobrick ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K143053 BBa_K143053]) has been characterised for expression of both GFPmut3b ([https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040]) and mRFP1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_E1010 BBa_E1010]). This data not only shows the parts are both working but once further parts have been characterized provides a basis for comparison. Further promoter and RBS combinations are currently being characterized, in addition to attempts for the construction of calibration curves to normalise the units of fluorescence to molecules of fluorescent proteins. |
Revision as of 01:24, 30 October 2008
Bacillus subtilis Key Parts
This page is aimed to highlight some of the key biobricks for B. subtilis that are available on the Registry of Standard Biological Parts.
AmyE Integration Biobricks
The AmyE integration bricks (BBa_K143001 and BBa_K143002 are composed of two biobricks that can be added to the 5' and 3' ends of a construct to allow integration into the B. subtilis genome. These biobricks have been successfully used within the constructs BBa_K143079 and BBa_K143082for integration. The possible advantages of integration are within stability and control of copy number.
Constitutive Promoters
The pVeg promoter and spoVG ribosome binding site (RBS) Biobrick (BBa_K143053) has been characterised for expression of both GFPmut3b (BBa_E0040) and mRFP1 (BBa_E1010). This data not only shows the parts are both working but once further parts have been characterized provides a basis for comparison. Further promoter and RBS combinations are currently being characterized, in addition to attempts for the construction of calibration curves to normalise the units of fluorescence to molecules of fluorescent proteins.