DNA

Part:BBa_K4361000:Design

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-08-21)


BlcR-binding oligo, 51 bp, scrambled


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-Ensure that this part is compatible with BioBrick standards -Ensure that no specific binding sites for BlcR are present in the sequence

Source

-Based off of Part:BBa_K4361001, which is naturally found in Agrobacterium tumefaciens.

References