Composite

Part:BBa_K4275014

Designed by: Zijian Wei   Group: iGEM22_GreatBay_SCIE   (2022-09-29)


pTDH3-Kluyveromyces marxianus alpha-mating factor secretion signal-NapBGS-T-tTDH1

1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 331
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 331
    Illegal NheI site found at 89
    Illegal NheI site found at 1777
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 331
    Illegal XhoI site found at 2852
    Illegal XhoI site found at 3923
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 331
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 331
    Illegal NgoMIV site found at 2349
    Illegal AgeI site found at 1363
    Illegal AgeI site found at 1381
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None