Regulatory

Part:BBa_K3844004:Sequence, Features, and Subparts

Designed by: Anya Sabhnani   Group: iGEM21_DNHS_SanDiego_CA   (2021-10-21)

1. Function

This is another transcription factor that also binds to the Styrene VOC. It mainly regulates and represses the transcription of certain genes.

2. Design Consideration

Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.

3. Characterization

Sequence:atgaccaccctggatagcaacaacaacaccggcggcgtgattacctatattggcagcagc ggcagcagcccgagccgcaccagcccggaaagcctgtatagcgataacagcaacggcagc tttcagagcctgacccagggctgcccgacctattttccgccgagcccgaccggcagcctg acccaggatccggcgcgcagctttggcagcattccgccgagcctgagcgatgatggcagc ccgagcagcagcagcagcagcagcagcagcagcagcagcttttataacggcagcccgccg ggcagcctgcaggtggcgatggaagatagcagccgcgtgagcccgagcaaaagcaccagc aacattaccaaactgaacggcatggtgctgctgtgcaaagtgtgcggcgatgtggcgagc ggctttcattatggcgtgcatgcgtgcgaaggctgcaaaggcttttttcgccgcagcatt cagcagaacattcagtataaacgctgcctgaaaaacgaaaactgcagcattgtgcgcatt aaccgcaaccgctgccagcagtgccgctttaaaaaatgcctgagcgtgggcatgagccgc gatgcggtgcgctttggccgcattccgaaacgcgaaaaacagcgcatgctggcggaaatg cagagcgcgatgaacctggcgaacaaccagctgagcagccagtgcccgctggaaaccagc ccgacccagcatccgaccccgggcccgatgggcccgagcccgccgccggcgccggtgccg agcccgctggtgggctttagccagtttccgcagcagctgaccccgccgcgcagcccgagc ccggaaccgaccgtggaagatgtgattagccaggtggcgcgcgcgcatcgcgaaattttt acctatgcgcatgataaactgggcagcagcccgggcaactttaacgcgaaccatgcgagc ggcagcccgccggcgaccaccccgcatcgctgggaaaaccagggctgcccgccggcgccg aacgataacaacaccctggcggcgcagcgccataacgaagcgctgaacggcctgcgccag gcgccgagcagctatccgccgacctggccgccgggcccggcgcatcatagctgccatcag agcaacagcaacggccatcgcctgtgcccgacccatgtgtatgcggcgccggaaggcaaa gcgccggcgaacagcccgcgccagggcaacagcaaaaacgtgctgctggcgtgcccgatg aacatgtatccgcatggccgcagcggccgcaccgtgcaggaaatttgggaagattttagc atgagctttaccccggcggtgcgcgaagtggtggaatttgcgaaacatattccgggcttt cgcgatctgagccagcatgatcaggtgaccctgctgaaagcgggcacctttgaagtgctg atggtgcgctttgcgagcctgtttaacgtgaaagatcagaccgtgatgtttctgagccgc accacctatagcctgcaggaactgggcgcgatgggcatgggcgatctgctgagcgcgatg tttgattttagcgaaaaactgaacagcctggcgctgaccgaagaagaactgggcctgttt accgcggtggtgctggtgagcgcggatcgcagcggcatggaaaacagcgcgagcgtggaa cagctgcaggaaaccctgctgcgcgcgctgcgcgcgctggtgctgaaaaaccgcccgctg gaaaccagccgctttaccaaactgctgctgaaactgccggatctgcgcaccctgaacaac Atgcatagcgaaaaactgctgagctttcgcgtggatgcgcag

4. References

1. https://www.genecards.org/cgi-bin/carddisp.pl?gene=NR1D1

2. https://www.ncbi.nlm.nih.gov/gene/9572

3. https://www.uniprot.org/uniprot/P20393

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 307
    Illegal PstI site found at 522
    Illegal PstI site found at 1513
    Illegal PstI site found at 1684
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 307
    Illegal PstI site found at 522
    Illegal PstI site found at 1513
    Illegal PstI site found at 1684
    Illegal NotI site found at 1283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 307
    Illegal PstI site found at 522
    Illegal PstI site found at 1513
    Illegal PstI site found at 1684
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 307
    Illegal PstI site found at 522
    Illegal PstI site found at 1513
    Illegal PstI site found at 1684
    Illegal NgoMIV site found at 765
    Illegal NgoMIV site found at 969
    Illegal NgoMIV site found at 1011
    Illegal NgoMIV site found at 1203
  • 1000
    COMPATIBLE WITH RFC[1000]