Regulatory

Part:BBa_K3844002:Sequence, Features, and Subparts

Designed by: Anya Sabhnani   Group: iGEM21_DNHS_SanDiego_CA   (2021-10-21)

1. Function

Also known as mimR, this is a transcriptional regulator. It specializes in multiple aspects of synthetic biology, including: ATP-binding, sequence-specific DNA binding, and transcription factor binding.

2. Design Consideration

Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.

3. Characterization

Sequence: atggtgcgccatgcggataccccgaccgatgcgcagcgcgcgcgcgaaca gtttctgagcaccggcgcgctgcagccggatgcggtggcgagcagcgtgctgaacagctg gcagcgcagccgcgaactgcatgtgcatccggatcgcgtggaactgccgtatgtgcgcga tccggataccgataccccgctgatgcatgcggcggcgccggtgctgcgccgcatggcgga agatctgagcgatcagagcgtgagcgtggtgctgaccagcgcggatggcctggtgctgga tcgcgtggcggcggataccgaatttgaacgcgtgctggatgatgtgcgcctggcgcgcgg ctatagctatgcggaagaatttgcgggcaccaacggcattggcaccgcgctggaaaccgg ccgcccggcgtttattcgcggcagcgaacattatgtgggcaccctgggcggcctggcgtg cgcgggcagcccgattcgcgaaccggtgacccgccgcattctgggcgtgattgatctgac ctgccgcgcgcgccaggcggatccgctgctgtttgtgctggcgaaaagcgcgggcagcca gattgaagatcgcctgcgcaccatgaacaacgaaaccgaaaccgcgctgctggatgcgta tctgaaacagagccgccgctatccgggcggcgtgctggcgattggcggcgatgtggtgct gatgaaccgctatctgcgccaggcgctggatgcggcggatcagaccgtgctgctggatca tgcggcggaactgacccgcagcagctttaccagcaccaccgtggcgcatctgccgagcgg cgcgagcgtgaaaattagcgcggcggaacgcattattgtgggcgtgcgcagcgatagcgt ggtgtttcatgtgagcgtgcatgtggcggaaagcattccggtgcgcagcagccagcatat tccgcgcctggcgggccagagcagcagctttcgccgcagcgcgcagcaggtggaacgctg ctatcgcgatcgcgaatgggtggtgattgaaggcgaacgcggcagcggccgcacctatct gggctatagcgtggtgcattttgtgaccccggaacgcagcattccggtgctgcatattga taactttgataccccggaaagctttgtggcggcgtttgaaagcgaaaccgatagcgcgga ttttgcggtgattgtgagcgatgtggatgaactgccggatgaagtgctgaacccgatggc ggcgattatgcagacccgcgcgggccgcggctggattgcggcgaccaccagcaccgaacg cgatagccagctggtggatctgctgatgctgccgttttttacccataccgtgaccgtgcc ggcgctgcgccatcgcattgaagatctgcaggaactggtgccgatgattctgaacgaact gagccgcggcgaagcgcgcatggatggcgaagcgatgcgccagctggcgaaactgccgtg gccgggcaacattgcgcagctgcgccatgtgctgaccgaaaccgtgcgccgccagcgcag cggcgtgattggcgtggataaactgccggcggaatgccgcagcgtgacccgccgcaaact gaccccgctggaagcgatggaacgcgatgcgattgtgcgcagcctgctggaaaacgatgg caacaaagcggatgcggcggcggcgctgggcatgagccgcgcgaccatttatcgcaaaat taaagattttggcattgcg

4. References

1. https://www.uniprot.org/uniprot/G3XGC1

2. https://www.uniprot.org/uniprot/A0QTU7.fasta

3. https://www.bioinformatics.org/sms2/rev_trans.html

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 70
    Illegal PstI site found at 1396
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 70
    Illegal PstI site found at 1396
    Illegal NotI site found at 1055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 231
    Illegal BglII site found at 1392
    Illegal BamHI site found at 549
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 70
    Illegal PstI site found at 1396
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 70
    Illegal PstI site found at 1396
    Illegal NgoMIV site found at 1368
    Illegal NgoMIV site found at 1575
    Illegal AgeI site found at 492
  • 1000
    COMPATIBLE WITH RFC[1000]