RNA

Part:BBa_K2810008:Design

Designed by: Ryan Coates   Group: iGEM18_Cardiff_Wales   (2018-08-31)


siRNA* from 5' region of Myzus persicae SP3 secreted protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is GoldenGate compatible and so has 5' and 3' sticky ends of 4bases to enable the part to ligate into pGBA2. It also has short 3 nucleotide sequences at each end, that when ligated into the pGBA2 plasmid, creates a recognition site for the Type II S restriction enzyme, BsaI.


Source

Myzus persicae genomic sequence

References