Plasmid

Part:BBa_K2751001:Design

Designed by: Hao-Tung Liu   Group: iGEM18_NYMU-Taipei   (2018-10-10)

pET32a-FPFSkel2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5167
    Illegal PstI site found at 1140
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5212
    Illegal PstI site found at 1140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5257
    Illegal XhoI site found at 5275
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5167
    Illegal PstI site found at 1140
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5167
    Illegal PstI site found at 1140
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3317
    Illegal NgoMIV site found at 3477
    Illegal NgoMIV site found at 5065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1314
    Illegal SapI.rc site found at 2396


Design Notes

Source

References