Part:BBa_C0012:Hard Information

BHSF 2020's characterization

After we decided to use toggle switch as the principle of our circuit, and use lac as a molecule in our circuit, we used these keywords to find the most suitable system for our yeast, and we found the article describing a hybrid promoter:

1.If a chimeric lac operator site (lacO) is positioned appropriately downstream of the TATA‐box, gene expression from a nmt::lacO promoter (hybrid promoter) can be greatly repressed by lacI inhibitor.

2.Insertion of the operator sequence that contains the lac operator into the nmt promoter does not interfere with the function of the promoter.

3.The addition of IPTG to the medium could relieve the repression to some extent, but it is not fully reversible. Kjaerulff S, Nielsen O. An IPTG-inducible derivative of the fission yeast nmt promoter. Yeast. 2015 Jun;32(6):469-78. doi: 10.1002/yea.3073. Epub 2015 Mar 31. Erratum in: Yeast. 2016 Jan;33(1):33. PMID: 25801050.