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- 17:47, 5 December 2016 Maddiehl (Talk | contribs) uploaded File:Time Course.png (We grew JM109 E. Coli cells that had been transformed with our plasmid in 0 to 15 mM KH2AsO4 concentrations for 12 hours to determine the time at which the cells began producing GFP. The total culture volume was 250uL with starting OD600 0.01 of cells...)