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• 13:21, 9 October 2022 Zeniazen5 (Talk | contribs) uploaded File:BBa K4214003 SDSPAGE.png (A production culture was set up using BL21 ''E. coli'' and induced with 0.5 mM IPTG once their ODs reached 0.5. The cultures were left overnight at 30°C for protein production. The cells were then harvested, pelleted and purified using a HisPur Cobalt...)