Regulatory
EGSH

Part:BBa_K415507

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)
Revision as of 02:25, 29 October 2010 by Joy (Talk | contribs)

pEGSH L4R1 MammoBlock Entry Vector

Figure 1. Schematic of PonS Induction of the EGSH promoter, leading to transcriptional activation.
Schematic: Response of K415507 to the addition of PonS, in the presence of VgEcR and RxR.

L4R1 MammoBlock entry vector. EGSH is an inducible promoter. In the presence of the proteins retinoid X receptor (RxR) and ecdysone receptors (VgEcR), the addition of ponasterone S (PonS) leads to complex formation of RxR, VgEcR, and PonS. This activated complex is then capable of inducing the EGSH promoter and allows for dramatic upregulation in transcriptional activity.

                                                                                                                                                                                                                        

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal BglII site found at 115
    Illegal BamHI site found at 663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 646


[edit]
Categories
Parameters
None