Part:BBa_K415506

pTRE-Tight L4R1 MammoBlock

Modified TRE promoter with six tetO sites to provide low basal transcriptional activity, a significant improvement over the previously used simple TRE promoter. Upon induction with rtTA or rtTA variants, and the addition of doxycycline (DOX), the transcriptional activity of TREt drastically increases.

Characterization

Figure 1. DOX Induction of TREt Promoter Post Calcium Phosphate Transfection of HEK293 rtTA3 Cell Line.

Response of K415506 to DOX induction.

DOX addition occured with medium change post calcium phosphate transfection of HEK293 rtTA3 cell line with TREt_EGFP construct.

Results DOX addition led to dramatic increase in transcriptional activity, as seen in Figure 1. Basal transcription level is shown to be low in the left panel.

                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  Illegal XbaI site found at 30
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  Illegal XbaI site found at 30
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 322
  Illegal XbaI site found at 30
  
• 1000
  COMPATIBLE WITH RFC[1000]