Composite

Part:BBa_K371019

Designed by: Yang Zhang, Ruijun Zhu   Group: iGEM10_USTC   (2010-10-11)
Revision as of 01:11, 29 October 2010 by Zocean (Talk | contribs)

pdu(Propanediol utilization)BMC shell gene under Plac control

This composite consists of eight pdu bacterial microcompartment(BMC) structure gene following a natural lac promotor(BBa_R0010). These genes come from four basic parts,pduAB,pduJK,pduN and pduTUV, which consist of the natural RBS and gene separately.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1894
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3236
  • 1000
    COMPATIBLE WITH RFC[1000]


In the expression of protein, we use and unite the method mentioned in the previous papers. [see protocol] The bacterial was induced by 1 mM IPTG at 30 ℃ for 4 hours. The results was as follows.[the one without pduN was a negative control used to prove the function of pduN: in the growth process, the one without pduN tends to grow with more deposit implying its possible role in the vertex]

Before iptg..jpg

We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet.

Pdu Sds-page.jpg

[edit]
Categories
Parameters
n/apdu(Propanediol utilization)BMC shell gene under Plac control