Coding

Part:BBa_K415505

Designed by: Joy Jiao, Laura Deming, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)
Revision as of 20:57, 28 October 2010 by Joy (Talk | contribs)

VgEcR_2A_RxR L1L2 MammoBlock Entry Vector

Mammalian L1L2 Entry Vector containing VgEcR_2A_RxR construct. VgEcR and RxR form a heterodimer to control expression of the EGSH promoter; they can be activated by the addition of the PonS compound. Here they are separated by a viral 2A site that allows for bicistronic expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2091
    Illegal PstI site found at 2272
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1585
    Illegal BamHI site found at 486
    Illegal BamHI site found at 2962
    Illegal BamHI site found at 3115
    Illegal BamHI site found at 3229
    Illegal XhoI site found at 1578
    Illegal XhoI site found at 1659
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
    Illegal NgoMIV site found at 1707
    Illegal NgoMIV site found at 2054
    Illegal NgoMIV site found at 3527
    Illegal AgeI site found at 2815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3000
    Illegal SapI.rc site found at 389
    Illegal SapI.rc site found at 521


[edit]
Categories
Parameters
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