Coding

Part:BBa_K415502

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)
Revision as of 20:37, 28 October 2010 by Ldeming (Talk | contribs)

SMAD5 L1L2 MammoBlock Entry Vector 

Smad5 is a transcription factor involved in the regulation of developmental pattern formation and adult homeostasis. It has been shown to act downstream of BMP2 to promote osteoblast differentiation. Cloned from human cDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal SpeI site found at 48
    Illegal SpeI site found at 891
    Illegal PstI site found at 243
    Illegal PstI site found at 1148
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal NheI site found at 609
    Illegal SpeI site found at 48
    Illegal SpeI site found at 891
    Illegal PstI site found at 243
    Illegal PstI site found at 1148
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal BglII site found at 225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal SpeI site found at 48
    Illegal SpeI site found at 891
    Illegal PstI site found at 243
    Illegal PstI site found at 1148
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 453
    Illegal EcoRI site found at 1422
    Illegal SpeI site found at 48
    Illegal SpeI site found at 891
    Illegal PstI site found at 243
    Illegal PstI site found at 1148
    Illegal AgeI site found at 5
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 813


[edit]
Categories
Parameters
None