Regulatory
CMV

Part:BBa_K415514

Designed by: Joy Jiao, Laura Deming, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)
Revision as of 19:18, 28 October 2010 by Ldeming (Talk | contribs)

pCMV L4R1 MammoBlock Entry Vector

MammoBlock L4R1 Promoter vector. Contains Human cytomegalovirus (CMV) promoter, one of the strongest promoters described. Based on RNA polymerase II system, CMV promoter drives higher-level constitutive expression of genes in a broader variety of mammalian cell lines, compared with RNA polymerase III based promoters such as U6 and H1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None