Regulatory

Part:BBa_K415513

Designed by: Joy Jiao, Laura Deming, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)
Revision as of 19:12, 28 October 2010 by Ldeming (Talk | contribs)

pWRE_SV40 L4R1 MammoBlock Entry Vector

L4R1 MammoBlock Promoter Vector. Contains the Wnt-Responsive Element from the Lef1 promoter, in front of the SV40 minimal promoter. Candidate mechanosensitive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal BglII site found at 155
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None