Coding

Part:BBa_K415504

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)
Revision as of 18:17, 28 October 2010 by Grantrob (Talk | contribs)

No part name specified with partinfo tag.

L1L2 Mammoblock entry vector containing the Rtta3_2a_hygro construct. rtTA3 is a DOX-inducible transcription factor controlling expression of the TRE promoter; Hygro is a selection marker against the Hygromycin antibiotic used to select for stable mammalian cell lines. They are separated by the viral 2A sequence which allows for polycistronic expression of genes on the same mRNA molecule.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal XbaI site found at 30
    Illegal PstI site found at 1462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal PstI site found at 1462
    Illegal NotI site found at 2170
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal BamHI site found at 339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal XbaI site found at 30
    Illegal PstI site found at 1462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1372
    Illegal EcoRI site found at 2178
    Illegal XbaI site found at 30
    Illegal PstI site found at 1462
    Illegal AgeI site found at 351
  • 1000
    COMPATIBLE WITH RFC[1000]


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