Part:BBa_K352011

pCooM Promoter coupled with RBS+RFP+Double terminator

pCooM Promoter coupled with RBS, RFP,double terminator

PcooF and PcooM, the two CooA-regulated R. rubrum promoters, contain 2-fold symmetric DNA sequences which are CooA-binding sites and are similar to the CRP consensus sequence. This similarity corralates with the tantamount helix-turn-helix motifs CooA and CRP. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo.

Imaging with confocal laser scanning microscopy

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 647
  Illegal AgeI site found at 759
  
• 1000
  COMPATIBLE WITH RFC[1000]