Device

Part:BBa_K352017:Design

Designed by: Ozkan Is   Group: iGEM10_METU_Turkey   (2010-10-27)
Revision as of 16:55, 28 October 2010 by Metugen (Talk | contribs)

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pCooM RBS RFP Double terminator CooA coupled with placI+RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
    Illegal AgeI site found at 759
    Illegal AgeI site found at 1617
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence information was acquired from NCBI and physical DNA of pcooM was synthesized from GENEART. Standart Biobrick assembly strategies(restriction digestion) were used to produce biobricks


Source

Hwan Youn, Robert L. Kerby, Mary Conrad, et al. 2004. Functionally Critical Elements of CooA-Related CO Sensors. J. Bacteriol. 186(5):1320-1329. doi:10.1128/JB.186.5.1320-1329.2004.

References


- Kevin M, Balazsi G, Collins J (2007). Combinatorial promoter design for engineering noisy gene expression. Proceedings of the National Academy of Sciences 2007 July 31; 104(31): 12726-31.



- He Y, Shelver D, Kerby R.L., and Roberts G.P. (1996). Characterization of a CO-responsive transcriptional activator from Rhodospirillum rubrum. The Journal of Biological Chemistry 1996; 271(5):120-123.


- Shelver D, Kerby R.L., He Y, and Roberts G.P. (1997). CooA, a CO-sensing transcription factor from Rhodospirillum rubrum, is a CO-binding heme protein. Proceedings of the National Academy of Sciences 1997; 94: 11216-20.


- Aono S, Ohkubo K, Matsuo T, and Nakajima T (1998). Redox-controlled ligand exchange of the Heme in the CO-sensing transcriptional activator CooA. The Journal of Biologycal Chemistry 1998; 273(40): 25757-64.