Reporter

Part:BBa_K352011:Design

Designed by: Ş.Serap Memik   Group: iGEM10_METU_Turkey   (2010-10-16)
Revision as of 15:50, 28 October 2010 by Metugen (Talk | contribs) (→‎Design Notes)

pCooM Promoter coupled with RBS+RFP+Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
    Illegal AgeI site found at 759
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Device created from a composite of parts.

Design Notes

The sequence information was acquired from NCBI and physical DNA of pcooM was synthesized from GENEART. Standart Biobrick assembly strategies(restriction digestion) were used to produce biobricks.

Source

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References


- Aono S, Ohkubo K, Matsuo T, and Nakajima T (1998). Redox-controlled ligand exchange of the Heme in the CO-sensing transcriptional activator CooA. The Journal of Biologycal Chemistry 1998; 273(40): 25757-64.


- Aono S (2003). Biochemical and Biophysical Properties of the CO-Sensing Transcriptional Activator CooA. Accounts of Chemical Research 2003; 36(11): 825-831.