Part:BBa_K352011:Experience

Signal Quantification of pCooM-RBS-RFP-TT-pLac-RBS-CooA:

We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.

The data shows that RFP fluorescence increases with CO induction.

Imaging with confocal laser scanning microscopy

Culturing CO Sensor E.coli with CO induction

We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.

User Reviews

There is a bit red color in flask without any CO exposure. This might be because cooA protein is transcriptional activator not a transcriptional factor. Therefore, a leakage have been observed in our experiments; however, there is very good differentiation which is sensible.

Reference: Aono S (2003). Biochemical and Biophysical Properties of the CO-Sensing Transcriptional Activator CooA. Accounts of Chemical Research 2003; 36(11): 825-831.

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