Device
Part:BBa_K352017:Experience
Designed by: Ozkan Is Group: iGEM10_METU_Turkey (2010-10-27)
Revision as of 13:58, 28 October 2010 by Metugen (Talk | contribs) (→Culturing CO Sensor E.coli with CO induction)
Signal Quantification of pCooM-RBS-RFP-TT-pLac-RBS-CooA:
We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.
The data shows that RFP fluorescence increases with CO induction.
Imaging with confocal laser scanning microscopy
Culturing CO Sensor E.coli with CO induction
We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.
Applications of BBa_K352017
User Reviews
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