Device

Part:BBa_K352017

Designed by: Ozkan Is   Group: iGEM10_METU_Turkey   (2010-10-27)
Revision as of 13:54, 28 October 2010 by Metugen (Talk | contribs)

pCooM RBS RFP Double terminator CooA coupled with placI+RBS

This part is formed by combination of pCooM Promoter coupled with RBS+RFP+Double terminator and CooA coupled with placI+RBS.

Agar plates: Our Agar plates with pCooM-RBS-RFP-TT-pLac-RBS-CooA showed that because of low level of constitutive binding of CooA to pCooM promoter, we have observed low level of RFP fluorescence on agar plates.


w7

w7

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
    Illegal AgeI site found at 759
    Illegal AgeI site found at 1617
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//classic/reporter
//function/reporter/fluorescence
Parameters
controlspecific soret peak @ OD420
emission510 nm
excitation395 nm
familycooA is member of cAMP receptor protein (CRP) and fumavate nitrate reduction (FNR)
functionRFP reporter synthesized when carbon monoxide binding cooA protein exists
keggcooA: avn:Avin_47010
ligandsCO bound cooA
n/apCooM RBS RFP Double terminator CooA coupled with placI+RBS
outputRFP
uniprotcooA: P72322