Plasmid_Backbone

Part:BBa_K305011:Design

Designed by: Maarten van den Nieuwenhof, Ramon Sieber, Arend Jan Suk   Group: iGEM10_Groningen   (2010-10-19)
Revision as of 04:03, 28 October 2010 by AJ (Talk | contribs) (→‎Design Notes)

Subtilin inducible expression plasmid for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3141
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3141
    Illegal BglII site found at 3060
    Illegal XhoI site found at 287
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3141
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3141
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3156
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 324


Design Notes

Contains a BamHI restriction site in between the prefix and suffix.


Cloning with this plasmid in certain E. coli strains results in the insertion of an IS element of around 800bp containing a PstI site.

Source

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References